YKL071W from Saccharomyces cerevisiae encodes a novel aldehyde reductase for detoxification of glycolaldehyde and furfural derived from lignocellulose.
Identifieur interne : 000A36 ( Main/Exploration ); précédent : 000A35; suivant : 000A37YKL071W from Saccharomyces cerevisiae encodes a novel aldehyde reductase for detoxification of glycolaldehyde and furfural derived from lignocellulose.
Auteurs : Hanyu Wang [République populaire de Chine] ; Yidan Ouyang [République populaire de Chine] ; Chang Zhou [République populaire de Chine] ; Difan Xiao [République populaire de Chine] ; Yaping Guo [République populaire de Chine] ; Lan Wu [République populaire de Chine] ; Xi Li [République populaire de Chine] ; Yunfu Gu [République populaire de Chine] ; Quanju Xiang [République populaire de Chine] ; Ke Zhao [République populaire de Chine] ; Xiumei Yu [République populaire de Chine] ; Likou Zou [République populaire de Chine] ; Menggen Ma [République populaire de Chine]Source :
- Applied microbiology and biotechnology [ 1432-0614 ] ; 2017.
Descripteurs français
- KwdFr :
- Acétaldéhyde (analogues et dérivés), Acétaldéhyde (métabolisme), Alcools (métabolisme), Aldose reductase (composition chimique), Aldose reductase (métabolisme), Antienzymes (métabolisme), Benzaldéhydes (métabolisme), Biotransformation (MeSH), Cinétique (MeSH), Concentration en ions d'hydrogène (MeSH), Formaldéhyde (métabolisme), Furfural (métabolisme), Lignine (composition chimique), Métaux (métabolisme), Oxidoreductases (composition chimique), Oxidoreductases (métabolisme), Oxydoréduction (MeSH), Protéines de Saccharomyces cerevisiae (composition chimique), Protéines de Saccharomyces cerevisiae (métabolisme), Régulation de l'expression des gènes fongiques (MeSH), Saccharomyces cerevisiae (enzymologie), Saccharomyces cerevisiae (métabolisme), Stabilité enzymatique (MeSH), Température (MeSH), Transcription génétique (MeSH).
- MESH :
- analogues et dérivés : Acétaldéhyde.
- composition chimique : Aldose reductase, Lignine, Oxidoreductases, Protéines de Saccharomyces cerevisiae.
- enzymologie : Saccharomyces cerevisiae.
- métabolisme : Acétaldéhyde, Alcools, Aldose reductase, Antienzymes, Benzaldéhydes, Formaldéhyde, Furfural, Métaux, Oxidoreductases, Protéines de Saccharomyces cerevisiae, Saccharomyces cerevisiae.
- Biotransformation, Cinétique, Concentration en ions d'hydrogène, Oxydoréduction, Régulation de l'expression des gènes fongiques, Stabilité enzymatique, Température, Transcription génétique.
English descriptors
- KwdEn :
- Acetaldehyde (analogs & derivatives), Acetaldehyde (metabolism), Alcohols (metabolism), Aldehyde Reductase (chemistry), Aldehyde Reductase (metabolism), Benzaldehydes (metabolism), Biotransformation (MeSH), Enzyme Inhibitors (metabolism), Enzyme Stability (MeSH), Formaldehyde (metabolism), Furaldehyde (metabolism), Gene Expression Regulation, Fungal (MeSH), Hydrogen-Ion Concentration (MeSH), Kinetics (MeSH), Lignin (chemistry), Metals (metabolism), Oxidation-Reduction (MeSH), Oxidoreductases (chemistry), Oxidoreductases (metabolism), Saccharomyces cerevisiae (enzymology), Saccharomyces cerevisiae (metabolism), Saccharomyces cerevisiae Proteins (chemistry), Saccharomyces cerevisiae Proteins (metabolism), Temperature (MeSH), Transcription, Genetic (MeSH).
- MESH :
- chemical , analogs & derivatives : Acetaldehyde.
- chemical , chemistry : Aldehyde Reductase, Lignin, Oxidoreductases, Saccharomyces cerevisiae Proteins.
- chemical , metabolism : Acetaldehyde, Alcohols, Aldehyde Reductase, Benzaldehydes, Enzyme Inhibitors, Formaldehyde, Furaldehyde, Metals, Oxidoreductases, Saccharomyces cerevisiae Proteins.
- enzymology : Saccharomyces cerevisiae.
- metabolism : Saccharomyces cerevisiae.
- Biotransformation, Enzyme Stability, Gene Expression Regulation, Fungal, Hydrogen-Ion Concentration, Kinetics, Oxidation-Reduction, Temperature, Transcription, Genetic.
Abstract
Aldehydes generated as by-products during the pretreatment of lignocellulose are the key inhibitors to Saccharomyces cerevisiae, which is considered as the most promising microorganism for industrial production of biofuel, xylitol as well as other special chemicals from lignocellulose. S. cerevisiae has the inherent ability to in situ detoxify aldehydes to corresponding alcohols by multiple aldehyde reductases. Herein, we report that an uncharacterized open reading frame YKL071W from S. cerevisiae encodes a novel "classical" short-chain dehydrogenase/reductase (SDR) protein with NADH-dependent enzymatic activities for reduction of furfural (FF), glycolaldehyde (GA), formaldehyde (FA), and benzaldehyde (BZA). This enzyme showed much better specific activities for reduction of GA and FF than FA and BZA, and displayed much higher Km and Kcat/Km but lower Vmax and Kcat for reduction of GA than FF. For this enzyme, the optimum pH was 5.5 and 6.0 for reduction of GA and FF, and the optimum temperature was 30 °C for reduction of GA and FF. Both pH and temperature affected stability of this enzyme in a similar trend for reduction of GA and FF. Cu2+, Zn2+, Ni2+, and Fe3+ had severe inhibition effects on enzyme activities of Ykl071wp for reduction of GA and FF. Transcription of YKL071W in S. cerevisiae was significantly upregulated under GA and FF stress conditions, and its transcription is most probably regulated by transcription factor genes of YAP1, CAD1, PDR3, and STB5. This research provides guidelines to identify more uncharacterized genes with reductase activities for detoxification of aldehydes derived from lignocellulose in S. cerevisiae.
DOI: 10.1007/s00253-017-8567-z
PubMed: 29034432
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Applied Microbiology, College of Resources, Sichuan Agricultural University, Wenjiang, 611130, Sichuan</wicri:regionArea><wicri:noRegion>Sichuan</wicri:noRegion></affiliation></author><author><name sortKey="Zou, Likou" sort="Zou, Likou" uniqKey="Zou L" first="Likou" last="Zou">Likou Zou</name><affiliation wicri:level="1"><nlm:affiliation>Department of Applied Microbiology, College of Resources, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Applied Microbiology, College of Resources, Sichuan Agricultural University, Wenjiang, 611130, Sichuan</wicri:regionArea><wicri:noRegion>Sichuan</wicri:noRegion></affiliation></author><author><name sortKey="Ma, Menggen" sort="Ma, Menggen" uniqKey="Ma M" first="Menggen" last="Ma">Menggen Ma</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Natural Resources and Geographic Information Technology, College of Resources, Sichuan Agricultural University, No. 211 Huimin Road, Wenjiang, 611130, Sichuan, People's Republic of China. mgen@sicau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Natural Resources and Geographic Information Technology, College of Resources, Sichuan Agricultural University, No. 211 Huimin Road, Wenjiang, 611130, Sichuan</wicri:regionArea><wicri:noRegion>Sichuan</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Applied Microbiology, College of Resources, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, People's Republic of China. mgen@sicau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Applied Microbiology, College of Resources, Sichuan Agricultural University, Wenjiang, 611130, Sichuan</wicri:regionArea><wicri:noRegion>Sichuan</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:29034432</idno><idno type="pmid">29034432</idno><idno type="doi">10.1007/s00253-017-8567-z</idno><idno type="wicri:Area/Main/Corpus">000A39</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000A39</idno><idno type="wicri:Area/Main/Curation">000A39</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000A39</idno><idno type="wicri:Area/Main/Exploration">000A39</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">YKL071W from Saccharomyces cerevisiae encodes a novel aldehyde reductase for detoxification of glycolaldehyde and furfural derived from lignocellulose.</title><author><name sortKey="Wang, Hanyu" sort="Wang, Hanyu" uniqKey="Wang H" first="Hanyu" last="Wang">Hanyu Wang</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Natural Resources and Geographic Information Technology, College of Resources, Sichuan Agricultural University, No. 211 Huimin Road, Wenjiang, 611130, Sichuan, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Natural Resources and Geographic Information Technology, College of Resources, Sichuan Agricultural University, No. 211 Huimin Road, Wenjiang, 611130, Sichuan</wicri:regionArea><wicri:noRegion>Sichuan</wicri:noRegion></affiliation></author><author><name sortKey="Ouyang, Yidan" sort="Ouyang, Yidan" uniqKey="Ouyang Y" first="Yidan" last="Ouyang">Yidan Ouyang</name><affiliation wicri:level="1"><nlm:affiliation>College of Environmental Sciences, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Environmental Sciences, Sichuan Agricultural University, Wenjiang, 611130, Sichuan</wicri:regionArea><wicri:noRegion>Sichuan</wicri:noRegion></affiliation></author><author><name sortKey="Zhou, Chang" sort="Zhou, Chang" uniqKey="Zhou C" first="Chang" last="Zhou">Chang Zhou</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Natural Resources and Geographic Information Technology, College of Resources, Sichuan Agricultural University, No. 211 Huimin Road, Wenjiang, 611130, Sichuan, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Natural Resources and Geographic Information Technology, College of Resources, Sichuan Agricultural University, No. 211 Huimin Road, Wenjiang, 611130, Sichuan</wicri:regionArea><wicri:noRegion>Sichuan</wicri:noRegion></affiliation></author><author><name sortKey="Xiao, Difan" sort="Xiao, Difan" uniqKey="Xiao D" first="Difan" last="Xiao">Difan Xiao</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Natural Resources and Geographic Information Technology, College of Resources, Sichuan Agricultural University, No. 211 Huimin Road, Wenjiang, 611130, Sichuan, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Natural Resources and Geographic Information Technology, College of Resources, Sichuan Agricultural University, No. 211 Huimin Road, Wenjiang, 611130, Sichuan</wicri:regionArea><wicri:noRegion>Sichuan</wicri:noRegion></affiliation></author><author><name sortKey="Guo, Yaping" sort="Guo, Yaping" uniqKey="Guo Y" first="Yaping" last="Guo">Yaping Guo</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Natural Resources and Geographic Information Technology, College of Resources, Sichuan Agricultural University, No. 211 Huimin Road, Wenjiang, 611130, Sichuan, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Natural Resources and Geographic Information Technology, College of Resources, Sichuan Agricultural University, No. 211 Huimin Road, Wenjiang, 611130, Sichuan</wicri:regionArea><wicri:noRegion>Sichuan</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Lan" sort="Wu, Lan" uniqKey="Wu L" first="Lan" last="Wu">Lan Wu</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Natural Resources and Geographic Information Technology, College of Resources, Sichuan Agricultural University, No. 211 Huimin Road, Wenjiang, 611130, Sichuan, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Natural Resources and Geographic Information Technology, College of Resources, Sichuan Agricultural University, No. 211 Huimin Road, Wenjiang, 611130, Sichuan</wicri:regionArea><wicri:noRegion>Sichuan</wicri:noRegion></affiliation></author><author><name sortKey="Li, Xi" sort="Li, Xi" uniqKey="Li X" first="Xi" last="Li">Xi Li</name><affiliation wicri:level="1"><nlm:affiliation>College of Landscape Architecture, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Landscape Architecture, Sichuan Agricultural University, Wenjiang, 611130, Sichuan</wicri:regionArea><wicri:noRegion>Sichuan</wicri:noRegion></affiliation></author><author><name sortKey="Gu, Yunfu" sort="Gu, Yunfu" uniqKey="Gu Y" first="Yunfu" last="Gu">Yunfu Gu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Applied Microbiology, College of Resources, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Applied Microbiology, College of Resources, Sichuan Agricultural University, Wenjiang, 611130, Sichuan</wicri:regionArea><wicri:noRegion>Sichuan</wicri:noRegion></affiliation></author><author><name sortKey="Xiang, Quanju" sort="Xiang, Quanju" uniqKey="Xiang Q" first="Quanju" last="Xiang">Quanju Xiang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Applied Microbiology, College of Resources, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Applied Microbiology, College of Resources, Sichuan Agricultural University, Wenjiang, 611130, Sichuan</wicri:regionArea><wicri:noRegion>Sichuan</wicri:noRegion></affiliation></author><author><name sortKey="Zhao, Ke" sort="Zhao, Ke" uniqKey="Zhao K" first="Ke" last="Zhao">Ke Zhao</name><affiliation wicri:level="1"><nlm:affiliation>Department of Applied Microbiology, College of Resources, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Applied Microbiology, College of Resources, Sichuan Agricultural University, Wenjiang, 611130, Sichuan</wicri:regionArea><wicri:noRegion>Sichuan</wicri:noRegion></affiliation></author><author><name sortKey="Yu, Xiumei" sort="Yu, Xiumei" uniqKey="Yu X" first="Xiumei" last="Yu">Xiumei Yu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Applied Microbiology, College of Resources, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Applied Microbiology, College of Resources, Sichuan Agricultural University, Wenjiang, 611130, Sichuan</wicri:regionArea><wicri:noRegion>Sichuan</wicri:noRegion></affiliation></author><author><name sortKey="Zou, Likou" sort="Zou, Likou" uniqKey="Zou L" first="Likou" last="Zou">Likou Zou</name><affiliation wicri:level="1"><nlm:affiliation>Department of Applied Microbiology, College of Resources, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Applied Microbiology, College of Resources, Sichuan Agricultural University, Wenjiang, 611130, Sichuan</wicri:regionArea><wicri:noRegion>Sichuan</wicri:noRegion></affiliation></author><author><name sortKey="Ma, Menggen" sort="Ma, Menggen" uniqKey="Ma M" first="Menggen" last="Ma">Menggen Ma</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Natural Resources and Geographic Information Technology, College of Resources, Sichuan Agricultural University, No. 211 Huimin Road, Wenjiang, 611130, Sichuan, People's Republic of China. mgen@sicau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Natural Resources and Geographic Information Technology, College of Resources, Sichuan Agricultural University, No. 211 Huimin Road, Wenjiang, 611130, Sichuan</wicri:regionArea><wicri:noRegion>Sichuan</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Applied Microbiology, College of Resources, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, People's Republic of China. mgen@sicau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Applied Microbiology, College of Resources, Sichuan Agricultural University, Wenjiang, 611130, Sichuan</wicri:regionArea><wicri:noRegion>Sichuan</wicri:noRegion></affiliation></author></analytic><series><title level="j">Applied microbiology and biotechnology</title><idno type="eISSN">1432-0614</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acetaldehyde (analogs & derivatives)</term><term>Acetaldehyde (metabolism)</term><term>Alcohols (metabolism)</term><term>Aldehyde Reductase (chemistry)</term><term>Aldehyde Reductase (metabolism)</term><term>Benzaldehydes (metabolism)</term><term>Biotransformation (MeSH)</term><term>Enzyme Inhibitors (metabolism)</term><term>Enzyme Stability (MeSH)</term><term>Formaldehyde (metabolism)</term><term>Furaldehyde (metabolism)</term><term>Gene Expression Regulation, Fungal (MeSH)</term><term>Hydrogen-Ion Concentration (MeSH)</term><term>Kinetics (MeSH)</term><term>Lignin (chemistry)</term><term>Metals (metabolism)</term><term>Oxidation-Reduction (MeSH)</term><term>Oxidoreductases (chemistry)</term><term>Oxidoreductases (metabolism)</term><term>Saccharomyces cerevisiae (enzymology)</term><term>Saccharomyces cerevisiae (metabolism)</term><term>Saccharomyces cerevisiae Proteins (chemistry)</term><term>Saccharomyces cerevisiae Proteins (metabolism)</term><term>Temperature (MeSH)</term><term>Transcription, Genetic (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acétaldéhyde (analogues et dérivés)</term><term>Acétaldéhyde (métabolisme)</term><term>Alcools (métabolisme)</term><term>Aldose reductase (composition chimique)</term><term>Aldose reductase (métabolisme)</term><term>Antienzymes (métabolisme)</term><term>Benzaldéhydes (métabolisme)</term><term>Biotransformation (MeSH)</term><term>Cinétique (MeSH)</term><term>Concentration en ions d'hydrogène (MeSH)</term><term>Formaldéhyde (métabolisme)</term><term>Furfural (métabolisme)</term><term>Lignine (composition chimique)</term><term>Métaux (métabolisme)</term><term>Oxidoreductases (composition chimique)</term><term>Oxidoreductases (métabolisme)</term><term>Oxydoréduction (MeSH)</term><term>Protéines de Saccharomyces cerevisiae (composition chimique)</term><term>Protéines de Saccharomyces cerevisiae (métabolisme)</term><term>Régulation de l'expression des gènes fongiques (MeSH)</term><term>Saccharomyces cerevisiae (enzymologie)</term><term>Saccharomyces cerevisiae (métabolisme)</term><term>Stabilité enzymatique (MeSH)</term><term>Température (MeSH)</term><term>Transcription génétique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Acetaldehyde</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Aldehyde Reductase</term><term>Lignin</term><term>Oxidoreductases</term><term>Saccharomyces cerevisiae Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Acetaldehyde</term><term>Alcohols</term><term>Aldehyde Reductase</term><term>Benzaldehydes</term><term>Enzyme Inhibitors</term><term>Formaldehyde</term><term>Furaldehyde</term><term>Metals</term><term>Oxidoreductases</term><term>Saccharomyces cerevisiae Proteins</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Acétaldéhyde</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Aldose reductase</term><term>Lignine</term><term>Oxidoreductases</term><term>Protéines de Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acétaldéhyde</term><term>Alcools</term><term>Aldose reductase</term><term>Antienzymes</term><term>Benzaldéhydes</term><term>Formaldéhyde</term><term>Furfural</term><term>Métaux</term><term>Oxidoreductases</term><term>Protéines de Saccharomyces cerevisiae</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biotransformation</term><term>Enzyme Stability</term><term>Gene Expression Regulation, Fungal</term><term>Hydrogen-Ion Concentration</term><term>Kinetics</term><term>Oxidation-Reduction</term><term>Temperature</term><term>Transcription, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biotransformation</term><term>Cinétique</term><term>Concentration en ions d'hydrogène</term><term>Oxydoréduction</term><term>Régulation de l'expression des gènes fongiques</term><term>Stabilité enzymatique</term><term>Température</term><term>Transcription génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Aldehydes generated as by-products during the pretreatment of lignocellulose are the key inhibitors to Saccharomyces cerevisiae, which is considered as the most promising microorganism for industrial production of biofuel, xylitol as well as other special chemicals from lignocellulose. S. cerevisiae has the inherent ability to in situ detoxify aldehydes to corresponding alcohols by multiple aldehyde reductases. Herein, we report that an uncharacterized open reading frame YKL071W from S. cerevisiae encodes a novel "classical" short-chain dehydrogenase/reductase (SDR) protein with NADH-dependent enzymatic activities for reduction of furfural (FF), glycolaldehyde (GA), formaldehyde (FA), and benzaldehyde (BZA). This enzyme showed much better specific activities for reduction of GA and FF than FA and BZA, and displayed much higher Km and Kcat/Km but lower Vmax and Kcat for reduction of GA than FF. For this enzyme, the optimum pH was 5.5 and 6.0 for reduction of GA and FF, and the optimum temperature was 30 °C for reduction of GA and FF. Both pH and temperature affected stability of this enzyme in a similar trend for reduction of GA and FF. Cu<sup>2+</sup>, Zn<sup>2+</sup>, Ni<sup>2+</sup>, and Fe<sup>3+</sup> had severe inhibition effects on enzyme activities of Ykl071wp for reduction of GA and FF. Transcription of YKL071W in S. cerevisiae was significantly upregulated under GA and FF stress conditions, and its transcription is most probably regulated by transcription factor genes of YAP1, CAD1, PDR3, and STB5. This research provides guidelines to identify more uncharacterized genes with reductase activities for detoxification of aldehydes derived from lignocellulose in S. cerevisiae.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29034432</PMID><DateCompleted><Year>2018</Year><Month>06</Month><Day>22</Day></DateCompleted><DateRevised><Year>2018</Year><Month>07</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-0614</ISSN><JournalIssue CitedMedium="Internet"><Volume>101</Volume><Issue>23-24</Issue><PubDate><Year>2017</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Applied microbiology and biotechnology</Title><ISOAbbreviation>Appl Microbiol Biotechnol</ISOAbbreviation></Journal><ArticleTitle>YKL071W from Saccharomyces cerevisiae encodes a novel aldehyde reductase for detoxification of glycolaldehyde and furfural derived from lignocellulose.</ArticleTitle><Pagination><MedlinePgn>8405-8418</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00253-017-8567-z</ELocationID><Abstract><AbstractText>Aldehydes generated as by-products during the pretreatment of lignocellulose are the key inhibitors to Saccharomyces cerevisiae, which is considered as the most promising microorganism for industrial production of biofuel, xylitol as well as other special chemicals from lignocellulose. S. cerevisiae has the inherent ability to in situ detoxify aldehydes to corresponding alcohols by multiple aldehyde reductases. Herein, we report that an uncharacterized open reading frame YKL071W from S. cerevisiae encodes a novel "classical" short-chain dehydrogenase/reductase (SDR) protein with NADH-dependent enzymatic activities for reduction of furfural (FF), glycolaldehyde (GA), formaldehyde (FA), and benzaldehyde (BZA). This enzyme showed much better specific activities for reduction of GA and FF than FA and BZA, and displayed much higher Km and Kcat/Km but lower Vmax and Kcat for reduction of GA than FF. For this enzyme, the optimum pH was 5.5 and 6.0 for reduction of GA and FF, and the optimum temperature was 30 °C for reduction of GA and FF. Both pH and temperature affected stability of this enzyme in a similar trend for reduction of GA and FF. Cu<sup>2+</sup>, Zn<sup>2+</sup>, Ni<sup>2+</sup>, and Fe<sup>3+</sup> had severe inhibition effects on enzyme activities of Ykl071wp for reduction of GA and FF. Transcription of YKL071W in S. cerevisiae was significantly upregulated under GA and FF stress conditions, and its transcription is most probably regulated by transcription factor genes of YAP1, CAD1, PDR3, and STB5. This research provides guidelines to identify more uncharacterized genes with reductase activities for detoxification of aldehydes derived from lignocellulose in S. cerevisiae.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Hanyu</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Institute of Natural Resources and Geographic Information Technology, College of Resources, Sichuan Agricultural University, No. 211 Huimin Road, Wenjiang, 611130, Sichuan, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ouyang</LastName><ForeName>Yidan</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>College of Environmental Sciences, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Chang</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Institute of Natural Resources and Geographic Information Technology, College of Resources, Sichuan Agricultural University, No. 211 Huimin Road, Wenjiang, 611130, Sichuan, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xiao</LastName><ForeName>Difan</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Institute of Natural Resources and Geographic Information Technology, College of Resources, Sichuan Agricultural University, No. 211 Huimin Road, Wenjiang, 611130, Sichuan, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Yaping</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Institute of Natural Resources and Geographic Information Technology, College of Resources, Sichuan Agricultural University, No. 211 Huimin Road, Wenjiang, 611130, Sichuan, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Lan</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Institute of Natural Resources and Geographic Information Technology, College of Resources, Sichuan Agricultural University, No. 211 Huimin Road, Wenjiang, 611130, Sichuan, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Xi</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>College of Landscape Architecture, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gu</LastName><ForeName>Yunfu</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Applied Microbiology, College of Resources, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xiang</LastName><ForeName>Quanju</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Department of Applied Microbiology, College of Resources, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Ke</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Applied Microbiology, College of Resources, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Xiumei</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Department of Applied Microbiology, College of Resources, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zou</LastName><ForeName>Likou</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Applied Microbiology, College of Resources, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Menggen</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institute of Natural Resources and Geographic Information Technology, College of Resources, Sichuan Agricultural University, No. 211 Huimin Road, Wenjiang, 611130, Sichuan, People's Republic of China. mgen@sicau.edu.cn.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Applied Microbiology, College of Resources, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, People's Republic of China. mgen@sicau.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>31570086</GrantID><Agency>National Natural Science Foundation of China</Agency><Country></Country></Grant><Grant><GrantID>01426100</GrantID><Agency>Sichuan Agricultural University</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>10</Month><Day>15</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Appl Microbiol Biotechnol</MedlineTA><NlmUniqueID>8406612</NlmUniqueID><ISSNLinking>0175-7598</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000438">Alcohols</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001547">Benzaldehydes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004791">Enzyme Inhibitors</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008670">Metals</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D029701">Saccharomyces cerevisiae Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>11132-73-3</RegistryNumber><NameOfSubstance UI="C036909">lignocellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>1HG84L3525</RegistryNumber><NameOfSubstance UI="D005557">Formaldehyde</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>DJ1HGI319P</RegistryNumber><NameOfSubstance UI="D005662">Furaldehyde</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.-</RegistryNumber><NameOfSubstance UI="C000626873">OSI1 protein, S cerevisiae</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.-</RegistryNumber><NameOfSubstance UI="D010088">Oxidoreductases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.1.1.21</RegistryNumber><NameOfSubstance UI="D000449">Aldehyde Reductase</NameOfSubstance></Chemical><Chemical><RegistryNumber>GO1N1ZPR3B</RegistryNumber><NameOfSubstance UI="D000079">Acetaldehyde</NameOfSubstance></Chemical><Chemical><RegistryNumber>TA269SD04T</RegistryNumber><NameOfSubstance UI="C032175">benzaldehyde</NameOfSubstance></Chemical><Chemical><RegistryNumber>W0A0XPU08U</RegistryNumber><NameOfSubstance UI="C010972">glycolaldehyde</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000079" MajorTopicYN="N">Acetaldehyde</DescriptorName><QualifierName UI="Q000031" MajorTopicYN="Y">analogs & derivatives</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000438" MajorTopicYN="N">Alcohols</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000449" MajorTopicYN="N">Aldehyde Reductase</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001547" MajorTopicYN="N">Benzaldehydes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001711" MajorTopicYN="N">Biotransformation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004791" MajorTopicYN="N">Enzyme Inhibitors</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004795" MajorTopicYN="N">Enzyme Stability</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005557" MajorTopicYN="N">Formaldehyde</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005662" MajorTopicYN="N">Furaldehyde</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015966" MajorTopicYN="N">Gene Expression Regulation, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006863" MajorTopicYN="N">Hydrogen-Ion Concentration</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007700" MajorTopicYN="N">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008670" MajorTopicYN="N">Metals</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010084" MajorTopicYN="N">Oxidation-Reduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010088" MajorTopicYN="N">Oxidoreductases</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012441" MajorTopicYN="N">Saccharomyces cerevisiae</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029701" MajorTopicYN="N">Saccharomyces cerevisiae Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013696" MajorTopicYN="N">Temperature</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014158" MajorTopicYN="N">Transcription, Genetic</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Aldehyde reductase</Keyword><Keyword MajorTopicYN="N">Detoxification</Keyword><Keyword MajorTopicYN="N">Enzyme activity</Keyword><Keyword MajorTopicYN="N">Saccharomyces cerevisiae</Keyword><Keyword MajorTopicYN="N">Short-chain dehydrogenase/reductase (SDR)</Keyword><Keyword MajorTopicYN="N">Transcriptional response</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>07</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>09</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2017</Year><Month>09</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>10</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>6</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>10</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29034432</ArticleId><ArticleId IdType="doi">10.1007/s00253-017-8567-z</ArticleId><ArticleId IdType="pii">10.1007/s00253-017-8567-z</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Hanyu" sort="Wang, Hanyu" uniqKey="Wang H" first="Hanyu" last="Wang">Hanyu Wang</name></noRegion><name sortKey="Gu, Yunfu" sort="Gu, Yunfu" uniqKey="Gu Y" first="Yunfu" last="Gu">Yunfu Gu</name><name sortKey="Guo, Yaping" sort="Guo, Yaping" uniqKey="Guo Y" first="Yaping" last="Guo">Yaping Guo</name><name sortKey="Li, Xi" sort="Li, Xi" uniqKey="Li X" first="Xi" last="Li">Xi Li</name><name sortKey="Ma, Menggen" sort="Ma, Menggen" uniqKey="Ma M" first="Menggen" last="Ma">Menggen Ma</name><name sortKey="Ma, Menggen" sort="Ma, Menggen" uniqKey="Ma M" first="Menggen" last="Ma">Menggen Ma</name><name sortKey="Ouyang, Yidan" sort="Ouyang, Yidan" uniqKey="Ouyang Y" first="Yidan" last="Ouyang">Yidan Ouyang</name><name sortKey="Wu, Lan" sort="Wu, Lan" uniqKey="Wu L" first="Lan" last="Wu">Lan Wu</name><name sortKey="Xiang, Quanju" sort="Xiang, Quanju" uniqKey="Xiang Q" first="Quanju" last="Xiang">Quanju Xiang</name><name sortKey="Xiao, Difan" sort="Xiao, Difan" uniqKey="Xiao D" first="Difan" last="Xiao">Difan Xiao</name><name sortKey="Yu, Xiumei" sort="Yu, Xiumei" uniqKey="Yu X" first="Xiumei" last="Yu">Xiumei Yu</name><name sortKey="Zhao, Ke" sort="Zhao, Ke" uniqKey="Zhao K" first="Ke" last="Zhao">Ke Zhao</name><name sortKey="Zhou, Chang" sort="Zhou, Chang" uniqKey="Zhou C" first="Chang" last="Zhou">Chang Zhou</name><name sortKey="Zou, Likou" sort="Zou, Likou" uniqKey="Zou L" first="Likou" last="Zou">Likou Zou</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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